PBX1 Rabbit Polyclonal Antibody
cat.: ER1914-79
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Zebrafish |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 47 KDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human PBX1 aa 1-250 / 430. |
| Positive control: | HeLa cell lysate, HEK-293 cell lysate, A431 cell lysate, NIH/3T3 cell lysate, Mouse testis tissue lysate, Rat pancreas tissue lysate, NIH/3T3. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IF-Cell FC |
1:2,000 1:2,000 1:1,000 |
| Uniprot #: | SwissProt: P40424 Human | P41778 Mouse Entrez Gene: 304947 Rat |
| Alternative names: | DKFZp686B09108 Homeo box protein PBX1 Homeo box protein PRL Homeobox protein PBX 1 Homeobox protein PBX1 Homeobox protein PRL MGC126627 PBX 1 Pbx1 PBX1_HUMAN Pre B cell leukemia homeobox 1 Pre B cell leukemia transcription factor 1 Pre-B-cell leukemia transcription factor 1 PRL |
Images
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Fig1:
Western blot analysis of PBX1 on different lysates with Rabbit anti-PBX1 antibody (ER1914-79) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: A431 cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: Mouse testis tissue lysate Lane 6: Rat pancreas tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 47 kDa Observed band size: 40/47 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1914-79) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of NIH/3T3 cells labeling PBX1 with Rabbit anti-PBX1 antibody (ER1914-79) at 1/2,000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PBX1 antibody (ER1914-79) at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Flow cytometric analysis of NIH/3T3 cells labeling PBX1. Cells were fixed and permeabilized. Then stained with the primary antibody (ER1914-79, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.