PDE4D Rabbit Polyclonal Antibody
cat.: ER1914-89
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | WB, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage buffer: | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 90 KDa |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human PDE4D 401-550/809 |
| Positive control: | Widespread; most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle. |
| Subcellular location: | Cytoplasm. Membrane. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, centrosome. Note=Found in the soluble fraction, associated with membranes, and associated with the cytoskeleton and the centrosome. |
| Recommended Dilutions:
WB FC |
1:500-2000 1μg/Test |
| Uniprot #: | SwissProt: Q08499 Human |
| Alternative names: | DPDE3 hPDE4D3 HSPDE4D PDE43 PDE4D PDE4DN2 Phosphodiesterase 4D Phosphodiesterase 4D cAMP-specific STRK1 |
Images
|
Fig1:
Sample:Brain(Mouse) Lysate at 30 ug Primary: Anti-PDE4D (bs-1325R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 90 kD Observed band size: 90kD |
|
Fig2:
Sample:Brain(Mouse) Lysate at 30 ug Primary: Anti-PDE4D (bs-1325R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 90 kD Observed band size: 100 kD |
|
Fig3:
Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti- PDE4D (bs-1325R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 90kD Observed band size: 90 kD |
|
Fig4:
Blank control: RSC96(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice.. Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μg in 100 μgL 1X PBS containing 0.5% BSA(green). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.