| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 53 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Presenilin 1 aa 1-50. |
| Positive control: | HeLa cell lysate, Neuro-2a cell lysate, Rat brain tissue lysate. |
| Subcellular location: | Endoplasmic reticulum, Endoplasmic reticulum membrane, Golgi apparatus membrane, Cytoplasmic granule, Cell membrane, Cell projection, growth cone, Early endosome, Early endosome membrane, Cell projection, neuron projection, Cell projection, axon, Synapse. |
| Recommended Dilutions:
WB |
1:2,000 |
| Uniprot #: | SwissProt: P49768 Human | P49769 Mouse | P97887 Rat |
| Alternative names: | AD3 Ad3h FAD Homo Sapiens Clone CC44 Senilin 1 Presenilin-1 CTF12 Protein S182 PS 1 PS-1 PS1-CTF12 PSEN1 PSN1_HUMAN PSNL1 S182 |
|
Fig1:
Western blot analysis of Presenilin 1 / PS-1 on different lysates with Rabbit anti-Presenilin 1 / PS-1 antibody (ER1915-40) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: Neuro-2a cell lysate Lane 3: Rat brain tissue lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 53 kDa Observed band size: 28 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1915-40) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |