ER1915-87

Product Type:	Rabbit polyclonal IgG, primary antibodies
Species reactivity:	Human, Mouse
Applications:	WB, IHC-P, IF-Tissue, FC
Clonality:	Polyclonal

Form:

Liquid

Storage condition:

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

Storage buffer:

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Concentration:

1ug/ul

Purification:

Protein A affinity purified.

Molecular weight:

60 KDa

Isotype:

IgG

Immunogen:

KLH conjugated synthetic peptide derived from human RIP2 401-540/540

Positive control:

Detected in heart, brain, placenta, lung, peripheral blood leukocytes, spleen, kidney, testis, prostate, pancreas and lymph node.

Subcellular location:

Cytoplasm.

Recommended Dilutions:
  WB
  IHC-P
  IF-tissue
  FC

1:500-2000
1:100-500
1:100-500
2ug/Test

Uniprot #:

SwissProt: O43353 Human

Alternative names:

CARD 3 CARD carrying kinase CARD containing ICE associated kinase CARD containing IL 1 beta ICE kinase CARD containing IL1 beta ICE kinase CARD containing interleukin 1 beta converting enzyme (ICE) associated kinase CARD containing interleukin 1 beta converting enzyme associated kinase CARD-containing IL-1 beta ICE-kinase CARD-containing interleukin-1 beta-converting enzyme-associated kinase CARD3 CARDIAK CCK CLARP kinase GIG 30 GIG30 Growth inhibiting gene 30 Receptor interacting protein (RIP) like interacting caspase like apoptosis regulatory protein (CLARP) kinase Receptor interacting protein 2 Receptor interacting serine threonine kinase 2 Receptor interacting serine/threonine protein kinase 2 Receptor-interacting protein 2 Receptor-interacting serine/threonine-protein kinase 2 RICK RIP 2 RIP like interacting CLARP kinase RIP-2 RIP-like-interacting CLARP kinase RIPK 2 Ripk2 RIPK2_HUMAN TNFRSF Tyrosine-protein kinase RIP......

	Fig1: Sample: Hela(Human) Cell Lysate at 30 ug Primary: Anti-RIP2 (bs-3546R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60 kD Observed band size: 60 kD
	Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RIP2) Polyclonal Antibody, Unconjugated (bs-3546R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
	Fig3: Blank control:A431. Primary Antibody (green line): Rabbit Anti-RIP2 antibody (bs-3546R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF488 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.