ER1916-44

Product Type:	Rabbit polyclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, IHC-P, IF-Tissue
Clonality:	Polyclonal

Form:

Liquid

Storage condition:

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

Storage buffer:

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Concentration:

1ug/ul

Purification:

Protein A affinity purified.

Molecular weight:

70 KDa

Isotype:

IgG

Immunogen:

KLH conjugated synthetic peptide derived from human 5-HTT 401-500/630

Positive control:

Expressed in platelets (at protein level).

Subcellular location:

Cell membrane; Multi-pass membrane protein. Endomembrane system; Multi-pass membrane protein. Endosome membrane; Multi-pass membrane protein.

Recommended Dilutions:
  WB
  IHC-P
  IF-tissue

1:500-2000
1:100-500
1:100-500

Uniprot #:

SwissProt: P31645 Human

Alternative names:

5 HTT 5 HTTLPR 5 hydroxytryptamine (serotonin) transporter 5 hydroxytryptamine transporter 5HT transporter 5HTT hSERT HTT Na+/Cl- dependent serotonin transporter OCD1 SC6A4_HUMAN Serotonin transporter 1 SERT SERT1 Slc6a4 Sodium dependent serotonin transporter Sodium-dependent serotonin transporter Solute carrier family 6 (neurotransmitter transporter) member 4 solute carrier family 6 (neurotransmitter transporter serotonin) member 4 Solute carrier family 6 member 4

	Fig1: Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-SLC6A4 (bs-1893R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 70 kD Observed band size: 70 kD
	Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (Serotonin transporter) Polyclonal Antibody, Unconjugated (bs-1893R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
	Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (Serotonin transporter) Polyclonal Antibody, Unconjugated (bs-1893R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.