ER1916-61

Product Type:	Rabbit polyclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, FC
Clonality:	Polyclonal

Form:

Liquid

Storage condition:

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

Storage buffer:

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Concentration:

1ug/ul

Purification:

Protein A affinity purified.

Molecular weight:

56 KDa

Isotype:

IgG

Immunogen:

KLH conjugated synthetic peptide derived from human CD172a 151-250/504

Positive control:

Ubiquitous. Highly expressed in brain. Detected on myeloid cells, but not T-cells. Detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas.

Subcellular location:

Membrane; Single-pass type I membraneprotein.

Recommended Dilutions:
WB
FC

1:500-2000
1μg /test

Uniprot #:

SwissProt: P78324 Human

Alternative names:

Signal regulatory protein alpha type 1 BIT Brain Ig like molecule with tyrosine based activation motifs Brain immunoglobulin like molecule with tyrosine based activation motifs CD172 antigen like family member A CD172a CD172a antigen Inhibitory receptor SHPS1 Macrophage fusion receptor MFR MYD 1 Myd 1 MYD1 p84 Protein tyrosine phosphatase non receptor type substrate 1 PTPNS1 SHP Substrate 1 SHPS1 Signal regulatory protein alpha 2 Signal regulatory protein alpha 3 Signal regulatory protein alpha Signal Regulatory Protein Alpha Type 1 Signal regulatory protein alpha type 2 SIRP SIRPA SIRPalpha SIRPalpha1 SIRPalpha2 SIRPalpha3 Tyrosine phosphatase SHP substrate 1 Tyrosine protein phosphatase non receptor type substrate 1 Tyrosine protein phosphatase non receptor type substrate CD172a.

	Fig1: Sample: Lane 1: Mouse Brain Lysates Lane 2: Mouse Cerebellum Lysates Lane 3: Mouse Spleen Lysates Lane 4: Mouse Placenta Lysates Lane 5: Rat Brain Lysates Lane 6: Rat Cerebellum Lysates Primary: Anti-SIRP Alpha(bs-2708R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56kDa Observed band size: 56kDa
	Fig2: Blank control(blue): U937 (fixed with 2% paraformaldehyde (10 min)). Primary Antibody:Rabbit Anti- SIRP antibody(bs-2708R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.