SREBP1 Rabbit Polyclonal Antibody
cat.: ER1917-19
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Tissue |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage buffer: | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 122 KDa |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human SREBP-1 301-450/1147 |
| Positive control: | Expressed in a wide variety of tissues, most abundant in liver and adrenal gland. In fetal tissues lung and liver shows highest expression. Isoform SREBP-1C predominates in liver, adrenal gland and ovary, whereas isoform SREBP-1A predominates in hepatoma cell lines. |
| Subcellular location: | Endoplasmic reticulum membrane. Nucleus. |
| Recommended Dilutions:
WB IHC-P IF-tissue |
1:500-2000 1:100-500 1:100-500 |
| Uniprot #: | SwissProt: P36956 Human |
| Alternative names: | ADD 1 bHLHd1 Class D basic helix-loop-helix protein 1 D630008H06 Processed sterol regulatory element-binding protein 1 SRBP1_HUMAN SREBF 1 SREBF1 SREBP 1 SREBP 1c SREBP-1 SREBP1 Sterol regulatory element binding protein 1 Sterol Regulatory Element Binding Transcription Factor 1 / Protein 1 Sterol regulatory element binding transcription factor 1 Sterol regulatory element-binding transcription factor 1 |
Images
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Fig1:
Sample: MCF-7(Human) Cell Lysate at 30 ug A549(Human) Cell Lysate at 30 ug Hela(Human) Cell Lysate at 30 ug SH-SY5Y(Human) Cell Lysate at 30 ug U87MG(Human) Cell Lysate at 30 ug A431(Human) Cell Lysate at 30 ug Primary: Anti-SREBP1 (bs-1402R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 124/65 kD Observed band size: 124 kD |
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Fig2: Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SREBP-1) Polyclonal Antibody, Unconjugated (bs-1402R) at 1:200 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. |
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Fig3: Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SREBP-1) Polyclonal Antibody, Unconjugated (bs-1402R) at 1:200 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. |
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Fig4: Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SREBP1) Polyclonal Antibody, Unconjugated (bs-1402R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. |
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Fig5: Paraformaldehyde-fixed, paraffin embedded (mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SREBP1) Polyclonal Antibody, Unconjugated (bs-1402R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. |
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Fig6: Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SREBP1) Polyclonal Antibody, Unconjugated (bs-1402R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.