TGF beta 3 Rabbit Polyclonal Antibody
cat.: ER1917-64
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Pig |
| Applications: | WB, IHC-P, IF-Tissue, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage buffer: | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 30/47 KDa |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human TGF-beta 3 41-90/412 |
| Positive control: | Pig bile duct, Mouse Placenta Lysate, Mouse Muscle Lysate. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IHC-P IF-tissue FC |
1:500-2000 1:100-500 1:100-500 1ug/Test |
| Uniprot #: | SwissProt: P10600 Human |
| Alternative names: | ARVD ARVD1 FLJ16571 LDS5 MGC105479 MGC118722 prepro-transforming growth factor beta-3 RNHF TGF beta 3 TGF beta3 TGF-beta-3 TGFB 3 Tgfb3 TGFB3_HUMAN transforming growth factor beta 3 Transforming growth factor beta-3 |
Images
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Fig1:
Sample: Lane 1: Mouse Prostate tissue lysates Lane 2: Mouse Placenta tissue lysates Lane 3: Rat Prostate tissue lysates Lane 4: Rat Placenta tissue lysates Lane 5: Human HepG2 cell lysates Lane 6: Human MCF-7 cell lysates Lane 7: Human SH-SY5Y cell lysates Primary: Anti-TGF beta 3 propeptide (bs-0099R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 30/47 kDa Observed band size: 47 kDa |
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Fig2:
Tissue/cell: pig bile duct; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-TGF-beta-3 Polyclonal Antibody, Unconjugated(bs-0099R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
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Fig3:
Blank control: SHSY5Y. Primary Antibody (green line): Rabbit Anti-TGF beta 3 propeptide antibody (bs-0099R) Dilution: 1ug/Test; Secondary Antibody : Goat anti-rabbit IgG-FITC Dilution: 0.5ug/Test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.