ER1917-65

Product Type:	Rabbit polyclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, IHC-P, IF-Tissue, FC
Clonality:	Polyclonal

Form:

Liquid

Storage condition:

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

Storage buffer:

0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Concentration:

1ug/ul

Purification:

Protein A affinity purified.

Molecular weight:

55 KDa

Isotype:

IgG

Immunogen:

KLH conjugated synthetic peptide derived from human TGF-beta R1 301-400/501

Positive control:

Found in all tissues examined, most abundant in placenta and least abundant in brain and heart.

Subcellular location:

Cell membrane; Single-pass type I membrane protein. Cell junction, tight junction.

Recommended Dilutions:
  WB
  IHC-P
  IF-tissue
  FC

1:500-2000
1:100-500
1:100-500
1ug/Test

Uniprot #:

SwissProt: P36897 Human

Alternative names:

AAT 5 AAT5 Activin A receptor type II like kinase 53kDa Activin A receptor type II like kinase Activin A receptor type II like kinase 53kD Activin A receptor type II like protein kinase of 53kD activin A receptor type II-like kinase 53kDa activin A receptor type II-like protein kinase of 53kD Activin receptor like kinase 5 Activin receptor-like kinase 5 ACVRLK 4 ACVRLK4 ALK 5 ALK-5 ALK5 LDS1A LDS2A MSSE Serine/threonine protein kinase receptor R4 Serine/threonine-protein kinase receptor R4 SKR 4 SKR4 TbetaR I TbetaR-I TGF beta receptor type 1 TGF beta receptor type I TGF beta type I receptor TGF-beta receptor type I TGF-beta receptor type-1 TGF-beta type I receptor TGFBR 1 TGFBR1 TGFBR1 protein TGFR 1 TGFR-1 TGFR1 TGFR1_HUMAN Transforming growth factor beta receptor 1 Transforming growth factor beta receptor I (activin A receptor type II like kinase 53kD) Transforming growth factor beta receptor I tr......

	Fig1: Sample: Lane 1: Placenta (Mouse) Lysate at 40 ug Lane 2: Small intestine (Rat) Lysate at 40 ug Primary: Anti- TGF beta Receptor I (bs-0638R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kD Observed band size: 58 kD
	Fig2: Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-TGF Beta R1/TGFBR1 Polyclonal Antibody, Unconjugated(bs-0638R) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
	Fig3: Blank control:U937. Primary Antibody (green line): Rabbit Anti-TGF beta Receptor I antibody (bs-0638R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.