| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Cow |
| Applications: | IHC-P, IF-Tissue |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage buffer: | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 62 kDa |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human TGF beta Receptor II: 241-330/567 |
| Positive control: | Kidney (Mouse) Lysate, Brain(Mouse) Lysate, Brain(Rat) Lysate, HepG2 Cell Lysate. |
| Subcellular location: | Cell membrane; Single-pass type I membrane protein. |
| Recommended Dilutions:
IHC-P IF-tissue |
1:100-500 1:100-500 |
| Uniprot #: | SwissProt: P37173 Human |
| Alternative names: | AAT3 FAA3 LDS1B LDS2 LDS2B MFS2 RIIC TAAD2 TbetaR II TbetaR-II TGF beta receptor type 2 TGF beta receptor type II TGF beta receptor type IIB TGF beta type II receptor TGF-beta receptor type II TGF-beta receptor type-2 TGF-beta type II receptor TGF-beta-R2 TGFB R2 TGFbeta - RII TGFbeta RII Tgfbr2 TGFR-2 TGFR2_HUMAN Transforming growth factor beta receptor II Transforming growth factor beta receptor type II Transforming growth factor beta receptor type IIC Transforming growth factor beta receptor II (70/80kDa) transforming growth factor beta receptor II alpha transforming growth factor beta receptor II beta transforming growth factor beta receptor II delta transforming growth factor beta receptor II epsilon transforming growth factor beta receptor II gamma Transforming growth factor-beta receptor type II |
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Fig1: Paraformaldehyde-fixed, paraffin embedded (mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (TGF beta Receptor II) Polyclonal Antibody, Unconjugated (bs-0117R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. |
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Fig2: Paraformaldehyde-fixed, paraffin embedded (rat placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (TGF beta Receptor II) Polyclonal Antibody, Unconjugated (bs-0117R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. |
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Fig3: Paraformaldehyde-fixed, paraffin embedded (human placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (TGF beta Receptor II) Polyclonal Antibody, Unconjugated (bs-0117R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. |
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Fig4: Paraformaldehyde-fixed, paraffin embedded (rat spleen); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (TGF beta Receptor II) Polyclonal Antibody, Unconjugated (bs-0117R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. |
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Fig5: Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (TGF beta Receptor II) Polyclonal Antibody, Unconjugated (bs-0117R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. |