TRAIL Rabbit Polyclonal Antibody
cat.: ER1917-99
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Tissue |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage buffer: | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 31 KDa |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human TRAIL 185-281/281 |
| Positive control: | Widespread; most predominant in spleen, lung and prostate. |
| Subcellular location: | Membrane; Single-pass type II membrane protein (Potential). |
| Recommended Dilutions:
WB IHC-P IF-tissue |
1:500-2000 1:100-500 1:100-500 |
| Uniprot #: | SwissProt: P50591 Human |
| Alternative names: | Apo 2 ligand APO 2L Apo-2 ligand Apo-2L APO2L CD253 CD253 antigen Chemokine tumor necrosis factor ligand superfamily member 10 Protein TRAIL TL2 TNF Related Apoptosis Inducing Ligand TNF related apoptosis inducing ligand TRAIL TNF-related apoptosis-inducing ligand TNF10_HUMAN TNFSF10 TRAIL Tumor necrosis factor (ligand) family member 10 Tumor Necrosis Factor (ligand) Superfamily Member 10 Tumor necrosis factor apoptosis inducing ligand splice variant delta Tumor necrosis factor ligand superfamily member 10 |
Images
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Fig1:
Sample: Lung (Mouse) Lysate at 30 ug U251 Cell Lysate at 30 ug Primary: rabbit Anti- TRAIL (bs-1214R) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1:5000 dilution; Predicted band size: 31 kD Observed band size: 26 kD |
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Fig2:
Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-TRAIL Polyclonal Antibody, Unconjugated(bs-1214R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
|
Fig3:
Tissue/cell: mouse spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-TRAIL Polyclonal Antibody, Unconjugated(bs-1214R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.