TREM1 Rabbit Polyclonal Antibody
cat.: ER1918-03
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IHC-P, IF-Tissue, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage buffer: | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 24 KDa |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human TREM1 151-234/234 |
| Positive control: | U937 cell, K562 Cell Lysate, human liver carcinoma. |
| Subcellular location: | Isoform 1: Cell membrane; Single-pass type I membrane protein (Potential). Isoform 2: Secreted (Potential). |
| Recommended Dilutions:
WB IHC-P IF-tissue FC |
1:500-2000 1:100-500 1:100-500 1μg/Test |
| Uniprot #: | SwissProt: Q9NP99 Human |
| Alternative names: | CD354 OTTMUSP00000018206 TREM 1 TREM-1 TREM1 TREM1_HUMAN Triggering receptor expressed on monocytes 1 Triggering receptor expressed on myeloid cells 1 Triggering receptor TREM 1 Triggering receptor TREM1 |
Images
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Fig1:
Sample: hcclm3(Human) Cell Lysate at 30 ug K562(Human) Cell Lysate at 30 ug Primary: Anti-TREM1 (bs-10306R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 24 kD Observed band size: 21 kD |
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Fig2: Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TREM1) Polyclonal Antibody, Unconjugated (bs-10306R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. |
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Fig3: Primary Antibody:Rabbit anti-TREM1 antibody Supplier catalog number: bs-10306R Supplier: Bioss Isotype Control Antibody: Rabbit IgG Secondary Antibody: F(ab’)2 fragment goat anti-rabbit IgG-PE Supplier: Jackson Lab. Positive control:U937(4% paraformaldehyde for 10 min at room temperature) |
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Fig4:
Primary Antibody:Rabbit anti-TREM1 antibody Supplier catalog number: bs-10306R Supplier: Bioss Isotype Control Antibody: Rabbit IgG Secondary Antibody: F(ab’)2 fragment goat anti-rabbit IgG-PE Supplier: Jackson Lab. Positive control:U937(4% paraformaldehyde for 10 min at room temperature) |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.