TRIM21 Rabbit Polyclonal Antibody
cat.: ER1918-07
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 54 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human TRIM21 aa 1-475 (P19474). |
| Positive control: | HeLa cell lysate, HeLa treated with 10ng/mL IFN-α for 16 hours cell lysate, A549 cell lysate, human tonsil tissue, human spleen tissue, mouse spleen tissue, rat spleen tissue. |
| Subcellular location: | Cytoplasm, Cytoplasmic vesicle, autophagosome, Nucleus, P-body. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:200-1:1,000 |
| Uniprot #: | SwissProt: P19474 Human | Q62191 Mouse Entrez Gene: 308901 Rat |
| Alternative names: | 52 kDa ribonucleoprotein autoantigen Ro/SS-A 52 kDa Ro protein 52kD Ro/SSA autoantigen Autoantigen Ro/SSA, 52-KD E3 ubiquitin-protein ligase TRIM21 RING finger protein 81 RNF81 Ro 52 Ro(SS-A) Ro52 RO52_HUMAN Sicca syndrome antigen A Sjoegren syndrome type A antigen Sjogren syndrome antigen A1 Sjogren syndrome type A antigen SS-A SSA SSA1: Sjogren syndrome antigen A1 (52kDa ribonucleoprotein autoantigen SS-A/Ro) TRIM21 Tripartite motif protein TRIM21 Tripartite motif-containing 21 Tripartite motif-containing protein 21 |
Images
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Fig1:
Western blot analysis of TRIM21 on different lysates with Rabbit anti-TRIM21 antibody (ER1918-07) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 10ng/mL IFN-α for 16 hours cell lysate Lane 3: A549-si NT cell lysate Lane 4: A549-si TRIM21 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 54 kDa Observed band size: 50 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1918-07) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-TRIM21 antibody (ER1918-07) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1918-07) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-TRIM21 antibody (ER1918-07) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1918-07) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-TRIM21 antibody (ER1918-07) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1918-07) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-TRIM21 antibody (ER1918-07) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1918-07) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.