uPA Receptor Rabbit Polyclonal Antibody
cat.: ER1918-43
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Tissue |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage buffer: | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 31 KDa |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human PLAUR 201-300/335 |
| Positive control: | Expressed in neurons of the rolandic area of the brain (at protein level). Expressed in the brain. |
| Subcellular location: | Isoform 1: Cell membrane; Lipid-anchor, GPI-anchor. Isoform 2: Secreted (Probable). |
| Recommended Dilutions:
WB IHC-P IF-tissue |
1:500-2000 1:100-500 1:100-500 |
| Uniprot #: | SwissProt: Q03405 Human |
| Alternative names: | CD 87 CD87 CD87 antigen MO 3 MO3 Monocyte activation antigen Mo3 Plasminogen activator receptor urokinase Plasminogen activator urokinase receptor PLAUR U PAR u plasminogen activator receptor U-PAR u-plasminogen activator receptor form 2 UPA receptor uPAR UPAR_HUMAN Urinary plasminogen activator receptor URKR Urokinase plasminogen activator receptor Urokinase plasminogen activator surface receptor urokinase-type plasminogen activator (uPA) receptor |
Images
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Fig1:
Sample: Lane 1: Cerebrum (Mouse) Tissue Lysate at 40 ug Lane 2: Cerebrum (Rat) Tissue Lysate at 40 ug Primary: Anti-uPA Receptor (bs-1927R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 31 kD Observed band size: 48 kD |
|
Fig2:
Sample: bone (Mouse) Lysate at 40 ug Primary: Anti-uPA Receptor (bs-1927R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 31 kD Observed band size: 48 kD |
|
Fig3:
Tissue/cell: mouse brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-uPA Receptor Polyclonal Antibody, Unconjugated(bs-1927R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
|
Fig4:
Tissue/cell: mouse adrenal gland; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-uPA Receptor Polyclonal Antibody, Unconjugated(bs-1927R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.