VLDL Receptor Rabbit Polyclonal Antibody
cat.: ER1918-54
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage buffer: | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 93 KDa |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human VLDL Receptor 551-650/873 |
| Positive control: | Abundant in heart and skeletal muscle; also ovary and kidney; not in liver. |
| Subcellular location: | Membrane; Single-pass type I membrane protein. Membrane, clathrin-coated pit; Single-pass type I membrane protein. |
| Recommended Dilutions:
WB IF-cell |
1:500-2000 1:100 |
| Uniprot #: | SwissProt: P98155 Human |
| Alternative names: | FLJ35024 Very low density lipoprotein receptor Very low-density lipoprotein receptor VLDL R VLDL receptor VLDL-R VLDLR VLDLR_HUMAN VLDLRCH |
Images
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Fig1:
Sample: Lane 1: A549 (Human) Cell Lysate at 30 ug Lane 2: 293T (Human) Cell Lysate at 30 ug Lane 3: A431 (Human) Cell Lysate at 30 ug Lane 4: SiHa (Human) Cell Lysate at 30 ug Primary: Anti-VLDL Receptor (bs-4228R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 120 kD Observed band size: 120 kD |
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Fig2: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (VLDL Receptor) polyclonal Antibody, Unconjugated (bs-4228R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.