TNF alpha Rabbit Polyclonal Antibody
cat.: ER1919-22
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 17/26 kDa |
| Isotype: | IgG |
| Immunogen: | KLH conjugated synthetic peptide derived from human TNF-alpha:181-233/233 <Extracellular> |
| Positive control: | Liver (mouse) Lysate, Diseased lung(mouse). |
| Subcellular location: | Cell membrane, Secreted. |
| Recommended Dilutions:
WB |
1:500-2000: 1:5000-10000 |
| Uniprot #: | SwissProt: P01375 Human | P06804 Mouse |
| Alternative names: | APC1 APC1 protein Cachectin DIF Differentiation inducing factor Macrophage cytotoxic factor Tnf TNF superfamily member 2 TNF superfamily member 2 TNF macrophage derived TNF monocyte derived TNF-a TNF-alpha TNFA TNFA_HUMAN TNFSF2 Tumor necrosis factor (TNF superfamily member 2) Tumor necrosis factor alpha Tumor necrosis factor Tumor necrosis factor ligand superfamily member 2 Tumor Necrosis Factor Membrane Form Tumor necrosis factor soluble form |
Images
|
Fig1:
Sample: Diseased lung(mouse) at 30ug; Primary: Anti-TNF alpha (bs-2081R) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G-HRP) at 1: 5000 dilution; Predicted band size :17/28 kD Observed band size :17/28 kD |
|
Fig2:
Sample: Liver (mouse) Lysate at 40 ug Primary: Anti-TNF alpha(bs-2081R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 17/26kD Observed band size: 25 kD |
|
Fig3:
Sample: Lane 1: A431 (Human) Cell Lysate at 30 ug Lane 2: Hela (Human) Cell Lysate at 30 ug Primary: Anti-TNF alpha (bs-2081R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36/17/26 kD Observed band size: 36 kD |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.