Optineurin Rabbit Polyclonal Antibody
cat.: ER2001-02
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 66 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human optineurin aa 1-40. |
| Positive control: | A431 cell lysates, human liver tissue, human kidney tissue, human placenta tissue, MCF-7. |
| Subcellular location: | Golgi apparatus, trans-Golgi network, Recycling endosome, perinuclear region, autophagosome, Cytoplasmic vesicle. |
| Recommended Dilutions:
WB FC |
1:500-1:1,000 1:50-1:100 |
| Uniprot #: | SwissProt: Q96CV9 Human |
| Alternative names: | 14.7K interacting protein Ag9 C5 ALS12 E3 14.7K interacting protein E3-14.7K-interacting protein FIP 2 FIP-2 FIP2 Glaucoma 1 open angle E (adult onset) Glaucoma 1 open angle E GLC1E HIP 7 HIP-7 HIP7 Huntingtin interacting protein 7 Huntingtin interacting protein HYPL Huntingtin interacting protein L Huntingtin yeast partner L Huntingtin-interacting protein 7 Huntingtin-interacting protein L HYPL Injury inducible protein I 55 NEMO related protein NEMO-related protein NRP Optic neuropathy inducing protein Optic neuropathy-inducing protein Optineurin OPTN OPTN_HUMAN TFIIIA IntP TFIIIA-IntP Transcription factor IIIA interacting protein Transcription factor IIIA-interacting protein Tumor necrosis factor alpha inducible cellular protein containing leucine zipper domains |
Images
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Fig1: Western blot analysis of Optineurin on A431 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-02, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
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Fig2: Flow cytometric analysis of Optineurin was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER2001-02, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.