| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 167 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human FYCO1 aa 1-200. |
| Positive control: | HeLa (Human cervical adenocarcinoma cell) cell lysate. |
| Subcellular location: | Lysosome, Endosome, autophagosome. |
| Recommended Dilutions:
WB |
1:5,000 |
| Uniprot #: | SwissProt: Q9BQS8 Human | Q8VDC1 Mouse Entrez Gene: 301085 Rat |
| Alternative names: | CATC2 CTRCT18 DKFZp779K1152 FLJ13335 FYCO1 FYCO1_HUMAN FYVE and coiled coil domain containing 1 FYVE and coiled coil domain containing protein 1 FYVE and coiled-coil domain-containing protein 1 MGC126517 MGC126519 RUFY3 RUN and FYVE domain containing 3 ZFYVE7 Zinc finger FYVE domain containing protein 7 Zinc finger FYVE domain-containing protein 7 |
|
Fig1:
Western blot analysis of FYCO1 on HeLa (Human cervical adenocarcinoma cell) cell lysate with Rabbit anti-FYCO1 antibody (ER2001-04) at 1/5,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 20 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER2001-04, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 167 kDa Observed band size: 167 kDa |