SAP97 Rabbit Polyclonal Antibody
cat.: ER2001-07
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 100 kDa. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human aa 1-100. |
| Positive control: | Siha cell lysate, A431 cell lysate, human liver carcinoma tissue, mouse colon tissue, A431. |
| Subcellular location: | Cell junction, Cell membrane, Cytoplasm, Endoplasmic reticulum, Membrane, Synapse. |
| Recommended Dilutions:
WB IHC-P FC |
1:500 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q12959 Human | Q811D0 Mouse | Q62696 Rat |
| Alternative names: | Discs large homolog 1 discs large, Drosophila, homolog of, 1 discs, large homolog 1 (Drosophila) Disks large homolog 1 dJ1061C18.1.1 DKFZp761P0818 DKFZp781B0426 DLG 1 DLG1 DLG1_HUMAN DLGH 1 DLGH1 hDlg Presynaptic protein SAP97 SAP 97 SAP-97 SAP97 Synapse associated protein 97 Synapse-associated protein 97 |
Images
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Fig1:
Western blot analysis of SAP97 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-07, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Siha cell lysate Lane 2: A431 cell lysate |
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Fig2: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-SAP97 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-07, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-SAP97 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-07, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Flow cytometric analysis of SAP97 was done on A431 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER2001-07, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.