LMO2 Rabbit Polyclonal Antibody
cat.: ER2001-08
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 18 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human LMO2 aa 1-50.
Positive control: K-562 cell lysate, U937 cell lysate, EA.hy926, RAW264.7, SW620, rat womb tissue, human tonsil tissue, human bone marrow tissue, human womb tissue, mouse brain tissue.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
1:500-1:1,000
1:50-1:100
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P25791 Human | P25801 Mouse
Entrez Gene: 362176 Rat
Alternative names: Cysteine rich protein TTG 2 Cysteine rich protein TTG2 Cysteine-rich protein TTG-2 LIM domain only 2 (rhombotin like 1) LIM domain only 2 LIM domain only protein 2 LMO 2 LMO-2 lmo2 RBTN 2 RBTN L1 RBTN2 RBTN2_HUMAN RBTNL 1 RBTNL1 RHOM 2 RHOM2 Rhombotin 2 Rhombotin like 1 Rhombotin-2 Rhombotin2 T cell translocation gene 2 T cell translocation protein 2 T-cell translocation protein 2 TTG 2 TTG2
Images
ER2001-08_1.jpg Fig1: Western blot analysis of LMO2 on different lysates with Rabbit anti-LMO2 antibody (ER2001-08) at 1/1,000 dilution.

Lane 1: K-562 cell lysate
Lane 2: U937 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 18 kDa
Observed band size: 18 kDa

Exposure time: 2 minutes 33 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER2001-08) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
ER2001-08_2.jpg Fig2: ICC staining of LMO2 in EA.hy926 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER2001-08, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ER2001-08_3.jpg Fig3: ICC staining of LMO2 in RAW264.7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER2001-08, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ER2001-08_4.jpg Fig4: ICC staining of LMO2 in SW620 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER2001-08, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ER2001-08_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat womb tissue using anti-LMO2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-08, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER2001-08_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-LMO2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-08, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER2001-08_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human bone marrow tissue using anti-LMO2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-08, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER2001-08_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded human womb tissue using anti-LMO2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-08, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER2001-08_9.jpg Fig9: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-LMO2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-08, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER2001-08_10.jpg Fig10: Flow cytometric analysis of LMO2 was done on SW620 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER2001-08, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.