RAB27B Rabbit Polyclonal Antibody
cat.: ER2001-10
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 25 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide with N-terminal Human RAB27B. |
| Positive control: | A549 cell lysates, SH-SY5Y. |
| Subcellular location: | Late endosome, Membrane, Lipid-anchor. |
| Recommended Dilutions:
WB FC |
1:5,000 1:500-1:1,000 |
| Uniprot #: | SwissProt: O00194 Human |
| Alternative names: | C25KG Rab27b RAB27B member RAS oncogene family RAS associated protein RAB27B Ras related protein Rab 27b Ras related protein Rab27b Ras-related protein Rab-27B RB27B_HUMAN Small GTP binding protein rab27b |
Images
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Fig1:
Western blot analysis of RAB27B on A549 cell lysate with Rabbit anti-RAB27B antibody (ER2001-10) at 1/5,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 30 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER2001-10, 1/5,000 in in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 25 kDa Observed band size: 25 kDa |
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Fig2: Flow cytometric analysis of RAB27B was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ER2001-10, 1ug/ml) (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.