CD35 Rabbit Polyclonal Antibody
cat.: ER2001-14
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 224 kDa. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Complement receptor type 1 aa 600-800. |
| Positive control: | HL-60 cell lysates, recombinant protein lysates, human spleen tissue, human kidney tissue, Daudi. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IHC-P FC |
1:500 1:50-1:200 1;50-1:100 |
| Uniprot #: | SwissProt: P17927 Human |
| Alternative names: | C3 binding protein C3b/C4b receptor C3BR C4BR CD 35 CD35 CD35 antigen complement component (3b/4b) receptor 1 (Knops blood group) complement component (3b/4b) receptor 1 including Knops blood group system Complement component receptor 1 Complement receptor 1 Complement receptor type 1 CR 1 CR1 CR1_HUMAN KN Knops blood group antigen |
Images
|
Fig1:
Western blot analysis of CD35 on different lysates with Rabbit anti-CD35 antibody (ER2001-14) at 1/1000 dilution. Lane 1: TF-1 cell lysate Lane 2: K-562 cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 224 kDa Observed band size: 280 kDa Exposure time: 1 minute; ECL: K1801; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER2001-14) at 1/50,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Western blot analysis of CD35 on HL-60 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-14, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
|
Fig3: Western blot analysis of CD35 on recombinant protein lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-14, 1/5000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
|
Fig4: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD35 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-14, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CD35 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-14, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig6: Flow cytometric analysis of CD35 was done on Daudi cells. The cells were fixed, permeabilized and stained with the primary antibody (ER2001-14, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.