KPTN Rabbit Polyclonal Antibody
cat.: ER2001-17
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 48 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within N-terminal human KPTN. |
| Positive control: | HepG2 cell lysate, SH-SY5Y cell lysate, rat brain tissue lysate, human placenta tissue, mouse testis tissue, SH-SY5Y. |
| Subcellular location: | Lysosome membrane, lamellipodium, stereocilium. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:2,000 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q96GT1 Human | Q9Y664 Human | Q8VCX6 Mouse |
| Alternative names: | 2E4 actin associated protein 2E4 actin binding protein Actin-associated protein 2E4 Kaptin Kptn KPTN protein KPTN_HUMAN |
Images
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Fig1:
Western blot analysis of KPTN on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-17, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell lysate Lane 2: SH-SY5Y cell lysate Lane 3: Rat brain tissue lysate |
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Fig2: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-KPTN antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-17, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-KPTN antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-17, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Flow cytometric analysis of KPTN was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ER2001-17, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.