DNA2 Rabbit Polyclonal Antibody
cat.: ER2001-20
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 120 kDa.
Isotype: IgG
Immunogen: Synthetic peptide within human DNA2 aa 960-1000.
Positive control: Human stomach tissue lysates, rat large intestine tissue, human liver tissue, human kidney tissue, mouse kidney tissue, HL-60.
Subcellular location: Mitochondrion, Nucleus.
Recommended Dilutions:
  WB
  IHC-P
  FC
1:500-1:1,000
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P51530 Human | Q6ZQJ5 Mouse | D3ZG52 Rat
Alternative names: DNA replication ATP-dependent helicase-like homolog DNA replication helicase 2 homolog DNA replication helicase 2, yeast, homolog of DNA replication helicase 2-like DNA replication helicase/nuclease 2 DNA2 DNA2-like helicase DNA2L DNA2L_HUMAN HDNA2 KIAA0083 PEOA6 SCKL8
Images
ER2001-20_1.jpg Fig1: Western blot analysis of DNA2 on human stomach tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-20, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ER2001-20_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat large intestine tissue using anti-DNA2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-20, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER2001-20_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-DNA2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-20, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER2001-20_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-DNA2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-20, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER2001-20_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-DNA2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-20, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER2001-20_6.jpg Fig6: Flow cytometric analysis of DNA2 was done on HL-60 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER2001-20, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.