GOLGA7 Rabbit Polyclonal Antibody
cat.: ER2001-22
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, IHC, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/ml.
Purification: Protein affinity purified.
Molecular weight: 16 kD
Isotype: IgG
Immunogen: Recombinant protein within Human GOLGA7 aa 1-300.
Positive control: Human stomach tissue lysate, A431 cell lysate, human breast cancer tissue, human kidney tissue, mouse kidney tissue, A431.
Subcellular location: Golgi apparatus, Membrane
Recommended Dilutions:
  WB
  IHC
  FC

1:500-1:1,000
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q7Z5G4 Human | Q91W53 Mouse
Alternative names: Golgin subfamily A member 7 Golgi complex-associated protein of 16 kDa GOLGA7
Images
ER2001-22_1.jpg Fig1: Western blot analysis of GOLGA7 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-22, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Human stomach tissue lysate
Lane 2: A431 cell lysate
ER2001-22_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-GOLGA7 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-22, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER2001-22_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-GOLGA7 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-22, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER2001-22_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-GOLGA7 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-22, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER2001-22_5.jpg Fig5: Flow cytometric analysis of GOLGA7 was done on A431 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER2001-22, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.