ER2001-24

ADAM22 Rabbit Polyclonal Antibody

cat.: ER2001-24

Product Type:	Rabbit polyclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, IHC-P, IF-Cell, FC
Clonality:	Polyclonal

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Immunogen affinity purified.
Molecular weight:	Predicted band size: 75 kDa (Mature Form).
Isotype:	IgG
Immunogen:	Synthetic peptide within human ADAM22 aa 700-750.
Positive control:	Mouse cerebellum tissue lysate, rat brain tissue tissue lysate, F9, N2A, SHG-44, rat cerebellum tissue, rat brain tissue, SHSY5Y.
Subcellular location:	Cell membrane, Cell projection, Membrane.
Recommended Dilutions: WB IHC-P IF-Cell FC	1:500-1:2,000 1:50-1:200 1:50-1:200 1:50-1:100
Uniprot #:	SwissProt: Q9P0K1 Human \| Q9R1V6 Mouse
Alternative names:	and cysteine-rich protein 2 Disintegrin and metalloproteinase domain-containing protein 22 disintegrin-like MDC 2 MDC2 Metalloproteinase disintegrin ADAM22 3 Metalloproteinase like disintegrin like and cysteine rich protein 2 Metalloproteinase-disintegrin ADAM22-3 Metalloproteinase-like MGC149832 A disintegrin and metalloproteinase domain 22 ADA22_HUMAN ADAM 22 ADAM metallopeptidase domain 22 adam22

Images

	Fig1: Western blot analysis of ADAM22 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-24, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse cerebellum tissue lysate Lane 2: Rat brain tissue tissue lysate
	Fig2: ICC staining of ADAM22 in F9 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER2001-24, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
	Fig3: ICC staining of ADAM22 in N2A cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER2001-24, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
	Fig4: ICC staining of ADAM22 in SHG-44 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER2001-24, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

	Fig5: Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-ADAM22 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ER2001-24, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig6: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ADAM22 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ER2001-24, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig7: Flow cytometric analysis of ADAM22 was done on SHSY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ER2001-24, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.