Flt3 Rabbit Polyclonal Antibody
cat.: ER2001-25
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, FC, IF-Cell
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 113 kDa.
Isotype: IgG
Immunogen: Synthetic peptide within mouse Flt3 aa 30-80.
Positive control: Rat testis lysates, 293T, rat testis tissue, human liver tissue, human spleen tissue, human placenta tissue, mouse brain tissue, THP-1.
Subcellular location: Endoplasmic reticulum lumen, membrane.
Recommended Dilutions:
  WB
  IHC-P
  FC
  IF-Cell
1:500-1:2,000
1:50-1:200
1:50-1:100
1:50
Uniprot #: SwissProt: P36888 Human | Q00342 Mouse
Alternative names: CD 135 CD135 CD135 antigen Fetal liver kinase 2 FL cytokine receptor Flk 2 Flk2 Flt 3 FLT-3 Flt3 FLT3_HUMAN FMS like tyrosine kinase 3 Fms related tyrosine kinase 3 Fms-like tyrosine kinase 3 Growth factor receptor tyrosine kinase type III Ly-72 OTTHUMP0000004234 Receptor type tyrosine protein kinase FLT3 Stem cell tyrosine kinase 1 Stk 1 STK-1 Stk1 Tyrosine protein kinase receptor FLT3 Tyrosine-protein kinase receptor FLT3
Images
ER2001-25_1.jpg Fig1: Western blot analysis of Flt3 on rat testis lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-25, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ER2001-25_2.jpg Fig2: ICC staining of Flt3 in 293T cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER2001-25, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ER2001-25_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Flt3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-25, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER2001-25_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Flt3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-25, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER2001-25_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Flt3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-25, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER2001-25_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Flt3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-25, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER2001-25_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Flt3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-25, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER2001-25_8.jpg Fig8: Flow cytometric analysis of Flt3 was done on THP-1 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER2001-25, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.