AKR1B1 Rabbit Polyclonal Antibody
cat.: ER2001-27
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Rat, Mouse
Applications: WB, IHC-P
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 36 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human AKR1B1 aa 1-50.
Positive control: Rat skeletal muscle tissue lysate, A549 cell lysate, rat seminal vesicle tissue, human seminal vesicle tissue.
Subcellular location: Cytoplasm.
Recommended Dilutions:
  WB
  IHC-P

1:500-1:1,000
1:100-1:500
Uniprot #: SwissProt: P15121 Human
Alternative names: ADR AKR1B 1 Akr1b1 Aldehyde reductase 1 Aldehyde reductase Aldo keto reductase family 1, member B1 Aldo-keto reductase family 1 member B1 aldo-keto reductase family 1, member B1 (aldose reductase) Aldose reductase aldr 1 ALDR_HUMAN aldr1 ALR2 AR Lii5 2 CTCL tumor antigen Low Km aldose reductase MGC1804
Images
ER2001-27_1.jpg Fig1: Western blot analysis of AKR1B1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-27, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Rat skeletal muscle tissue lysate
Lane 2: A549 cell lysate
ER2001-27_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat seminal vesicle tissue using anti-AKR1B1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-27, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER2001-27_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human seminal vesicle tissue using anti-AKR1B1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-27, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.