COPS8 Rabbit Polyclonal Antibody
cat.: ER2001-29
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 23 kDa.
Isotype: IgG
Immunogen: Recombinant protein within human COPS8 aa 1-300.
Positive control: SHSY5Y cell lysate, HL-60 cell lysate, rat brain tissue, rat cerebellum tissue, human liver tissue, human liver carcinoma tissue, mouse brain tissue.
Subcellular location: Cytoplasm, Nucleus, Signalosome.
Recommended Dilutions:
  WB
  IHC-P
1:500-1:1,000
1:100-1:500
Uniprot #: SwissProt: Q99627 Human | Q8VBV7 Mouse | Q6P4Z9 Rat
Alternative names: COP9 homolog COP9 signalosome complex subunit 8 COP9 signalosome subunit 8 Cops8 CSN8 CSN8_HUMAN hCOP9 JAB1 containing signalosome subunit 8 JAB1-containing signalosome subunit 8 SGN8 Signalosome subunit 8
Images
ER2001-29_1.jpg Fig1: Western blot analysis of COPS8 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-29, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: SHSY5Y cell lysate
Lane 2: HL-60 cell lysate
ER2001-29_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-COPS8 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-29, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER2001-29_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-COPS8 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-29, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER2001-29_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-COPS8 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-29, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER2001-29_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-COPS8 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-29, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER2001-29_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-COPS8 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-29, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.