Anti-EPHA2 antibody
cat.: ER2001-31
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC, IHC, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/ml.
Purification: Peptide affinity purified.
Molecular weight: Predicted band size: 108 kDa.
Isotype: IgG
Immunogen: Synthetic peptide within human EPHA2 aa 550-600.
Positive control: A549 cell lysate, PC-3M cell lysate, PANC-1, rat brain tissue, mouse brain tissue, SHSY5Y.
Subcellular location: Cell junction, Cell membrane, Cell projection, Membrane.
Recommended Dilutions:
  WB
  ICC
  IHC
  FC

1:500-1:2,000
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P29317 Human | Q03145 Mouse
Alternative names: ARCC2 antibody AW545284 antibody CTPA antibody CTPP1 antibody CTRCT6 antibody EC 2.7.10.1 antibody Eck antibody Eph receptor A2 antibody EPHA2 antibody EPHA2_HUMAN antibody Ephrin receptor antibody Ephrin receptor EphA2 antibody Ephrin type A receptor 2 antibody Ephrin type-A receptor 2 antibody Epithelial cell kinase antibody Epithelial cell receptor protein tyrosine kinase antibody Myk 2 antibody Myk2 antibody Sek 2 antibody Sek2 antibody Soluble EPHA2 variant 1 antibody Tyrosine protein kinase receptor ECK antibody Tyrosine-protein kinase receptor ECK antibody Tyrosine-protein kinase receptor MPK-5 antibody Tyrosine-protein kinase receptor SEK-2 antibody
Images
ER2001-31_1.jpg Fig1: Western blot analysis of EPHA2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-31, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: A549 cell lysate
Lane 2: PC-3M cell lysate
ER2001-31_2.jpg Fig2: ICC staining of EPHA2 in PANC-1 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER2001-31, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ER2001-31_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-EPHA2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-31, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER2001-31_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-EPHA2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-31, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER2001-31_5.jpg Fig5: Flow cytometric analysis of EPHA2 was done on SHSY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ER2001-31, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.