EphA2 (N-term) Rabbit Polyclonal Antibody
cat.: ER2001-31
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at 4℃ short term. Aliquot and store at -20℃ long term. Avoid freeze/thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 108 kDa. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human EPHA2 aa 550-600. |
| Subcellular location: | Cell junction, Cell membrane, Cell projection, Membrane. |
| Recommended Dilutions:
WB IHC-P FC |
1:1,000 1:100-1:500 1:10-1:50 |
| Uniprot #: | SwissProt: P29317 Human | Q03145 Mouse |
| Alternative names: | ARCC2 AW545284 CTPA CTPP1 CTRCT6 EC 2.7.10.1 Eck Eph receptor A2 EPHA2 EPHA2_HUMAN Ephrin receptor Ephrin receptor EphA2 Ephrin type A receptor 2 Ephrin type-A receptor 2 Epithelial cell kinase Epithelial cell receptor protein tyrosine kinase Myk 2 Myk2 Sek 2 Sek2 Soluble EPHA2 variant 1 Tyrosine protein kinase receptor ECK Tyrosine-protein kinase receptor ECK Tyrosine-protein kinase receptor MPK-5 Tyrosine-protein kinase receptor SEK-2 |
Images
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Fig1: Anti-EphA2 (N-term) Antibody (ER2001-31) at 1:1,000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10,000 dilution. Predicted band size : 108 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
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Fig2: Western blot analysis of EphA2 (N-term) (Cat. #ER2001-31) in MCF-7 cell line lysates (35ug/lane). EphA2 (N-term) (arrow) was detected using the purified Pab. |
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Fig3: Western blot analysis of EphA2 (N-term) (Cat. #ER2001-31) in mouse NIH-3T3 cell line lysates (35ug/lane). EphA2 (N-term) (arrow) was detected using the purified Pab. |
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Fig4: Flow cytometric analysis of NCI-H292 cells using EphA2 (N-term) Antibody (bottom histogram) (ER2001-31) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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Fig5: Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody (ER2001-31), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.