Androgen Receptor Rabbit Polyclonal Antibody
cat.: ER2001-34
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Rat
Applications: WB, ICC, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 99 kDa
Isotype: IgG
Immunogen: Recombinant protein within human Androgen Receptor aa 200-500.
Positive control: Human skeletal muscle tissue lysate, rat heart tissue lysate, A549, MCF-7.
Subcellular location: Cytoplasm, Nucleus
Recommended Dilutions:

Uniprot #: SwissProt: P10275 Human | P15207 Rat
Alternative names: AIS ANDR_HUMAN Androgen nuclear receptor variant 2 Androgen receptor (dihydrotestosterone receptor; testicular feminization; spinal and bulbar muscular atrophy; Kennedy disease) Androgen receptor androgen receptor splice variant 4b AR AR8 DHTR Dihydro testosterone receptor Dihydrotestosterone receptor (DHTR) Dihydrotestosterone receptor HUMARA HYSP1 KD Kennedy disease (KD) NR3C4 Nuclear receptor subfamily 3 group C member 4 (NR3C4) Nuclear receptor subfamily 3 group C member 4 SBMA SMAX1 Spinal and bulbar muscular atrophy (SBMA) Spinal and bulbar muscular atrophy Testicular Feminization (TFM) TFM
ER2001-34_1.jpg Fig1: Western blot analysis of Androgen Receptor on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-34, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Human skeletal muscle tissue lysate
Lane 2: Rat heart tissue lysate
ER2001-34_2.jpg Fig2: ICC staining of Androgen Receptor in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER2001-34, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
ER2001-34_3.jpg Fig3: Flow cytometric analysis of Androgen Receptor was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER2001-34, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).