P2RX5 Rabbit Polyclonal Antibody
cat.: ER2001-36
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Rat, Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 51 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within rat P2RX5 aa 400-455. |
| Positive control: | Rat brain tissue lysate, rat heart tissue lysate, mouse brain tissue, rat brain tissue. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:1,000 1:50-1:200 |
| Uniprot #: | SwissProt: P51578 Rat | Q3UYI1 Mouse |
| Alternative names: | ATP receptor ATP receptor subunit Ionotropic ATP receptor P2X5 LRH 1 LRH1 Lymphoid restricted histocompatibility antigen 1 MGC47755 P2rx5 P2RX5_HUMAN P2X purinoceptor 5 P2X5 P2X5R Purinergic receptor Purinergic receptor P2X ligand gated ion channel 5 Purinergic receptor P2X5 |
Images
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Fig1:
Western blot analysis of P2RX5 on 10% mouse heart lysate with Rabbit anti-P2RX5 antibody (ER2001-36) at 1/1,000 dilution Exposure time: 60 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER2001-36, 1/1,000 in 5% BSA, 2 hours at room temperature Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1:5,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 51 kDa Observed band size: 51 kDa |
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Fig2: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-P2RX5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-36, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-P2RX5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-36, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.