TMEM119 Rabbit Polyclonal Antibody
cat.: ER2001-37
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB
Clonality: Polyclonal
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 29 kDa.
Isotype: IgG
Immunogen: Synthetic peptide within human Transmembrane protein 119 aa 120-170.
Positive control: SH-SY5Y (Human neuroblastoma cell) cell lysate, Neuro-2a (Mouse brain neuroblastoma cell) cell lysate, C6 (Rat glioma cell) cell lysate.
Subcellular location: Cell membrane, Cytoplasm, Endoplasmic reticulum, Membrane.
Recommended Dilutions:
  WB

1:500-1:1,000
Uniprot #: SwissProt: Q4V9L6 Human
Alternative names: OBIF Osteoblast induction factor PSEC0199 Transmembrane protein 119 UNQ731/PRO1415
Images
ER2001-37_1.jpg Fig1: Western blot analysis of TMEM119 on different lysates with Rabbit anti-TMEM119 antibody (ER2001-37) at 1/2,000 dilution.

Lane 1: SH-SY5Y (Human neuroblastoma cell) cell lysate
Lane 2: Neuro-2a (Mouse brain neuroblastoma cell) cell lysate
Lane 3: C6 (Rat glioma cell) cell lysate

Lysates/proteins at 20 µg/Lane.
Exposure time: 1 minute 2 seconds; ECL: K1801

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ER2001-37, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 29 kDa
Observed band size: 35 kDa
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.