| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 29 kDa. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Transmembrane protein 119 aa 120-170. |
| Positive control: | SH-SY5Y (Human neuroblastoma cell) cell lysate, Neuro-2a (Mouse brain neuroblastoma cell) cell lysate, C6 (Rat glioma cell) cell lysate. |
| Subcellular location: | Cell membrane, Cytoplasm, Endoplasmic reticulum, Membrane. |
| Recommended Dilutions:
WB |
1:500-1:1,000 |
| Uniprot #: | SwissProt: Q4V9L6 Human |
| Alternative names: | OBIF Osteoblast induction factor PSEC0199 Transmembrane protein 119 UNQ731/PRO1415 |
|
Fig1:
Western blot analysis of TMEM119 on different lysates with Rabbit anti-TMEM119 antibody (ER2001-37) at 1/2,000 dilution. Lane 1: SH-SY5Y (Human neuroblastoma cell) cell lysate Lane 2: Neuro-2a (Mouse brain neuroblastoma cell) cell lysate Lane 3: C6 (Rat glioma cell) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 1 minute 2 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER2001-37, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 29 kDa Observed band size: 35 kDa |