| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 145 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human mSin3A aa 1-50. |
| Positive control: | THP-1 cell lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB |
1:500-1:5,000 |
| Uniprot #: | SwissProt: Q96ST3 Human |
| Alternative names: | AW553200 DKFZP434K2235 FLJ90319 Histone deacetylase complex subunit Sin 3a Histone deacetylase complex subunit Sin3a KIAA0700 Kiaa4126 mKIAA4126 Paired amphipathic helix protein Sin 3a Paired amphipathic helix protein Sin3a Sin 3a SIN3 homolog A SIN3 homolog A transcription regulator (yeast) SIN3 homolog A transcription regulator SIN3 transcription regulator homolog A Sin3a SIN3A protein SIN3A_HUMAN Transcriptional co repressor Sin 3A Transcriptional co repressor Sin3A Transcriptional corepressor Sin3a Transcriptional regulator SIN3A |
|
Fig1:
Western blot analysis of mSin3A on different lysates with Rabbit anti-mSin3A antibody (ER2001-38) at 1/5000 dilution. Lane 1: THP-1 (Human acute monoblastic leukemia cell) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 3 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER2001-38, 1/5000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 145.1 kDa Observed band size: 170 kDa |