CLIC2 Rabbit Polyclonal Antibody
cat.: ER2001-41
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 28 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human CLIC2 aa 80-247. |
| Positive control: | K562 cell lysate, mouse lung tissue lysate, K562. |
| Subcellular location: | Cytoplasm, Membrane. |
| Recommended Dilutions:
WB FC |
1:500-1:1,000 1:50-1:100 |
| Uniprot #: | SwissProt: O15247 Human | Q5M883 Rat |
| Alternative names: | Chloride intracellular channel 2 Chloride intracellular channel protein 2 CLIC 2b CLIC2 CLIC2_HUMAN CLIC2b MRXS32 OTTHUMP00000196471 XAP 121 XAP121 |
Images
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Fig1: Flow cytometric analysis of CLIC2 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER2001-41, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
|
Fig2:
Western blot analysis of CLIC2 on different lysates with Rabbit anti-CLIC2 antibody (ER2001-41) at 1/2,000 dilution. Lane 1: K-562 (Human chronic myelogenous leukemia cells) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 30 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER2001-41, 1/2,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 28 kDa Observed band size: 28 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.