CLIC2 Rabbit Polyclonal Antibody
cat.: ER2001-41
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/ml.
Purification: Immunogen affinity purified.
Molecular weight: 28 kDa
Isotype: IgG
Immunogen: Recombinant protein within human CLIC2 aa 80-247.
Positive control: K562 cell lysate, mouse lung tissue lysate, K562.
Subcellular location: Cytoplasm, Membrane.
Recommended Dilutions:
  WB
  FC

1:500-1:1,000
1:50-1:100
Uniprot #: SwissProt: O15247 Human | Q5M883 Rat
Alternative names: Chloride intracellular channel 2 Chloride intracellular channel protein 2 CLIC 2b CLIC2 CLIC2_HUMAN CLIC2b MRXS32 OTTHUMP00000196471 XAP 121 XAP121
Images
ER2001-41_1.jpg Fig1: Western blot analysis of CLIC2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-41, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: K562 cell lysate
Lane 2: Mouse lung tissue lysate
ER2001-41_2.jpg Fig2: Flow cytometric analysis of CLIC2 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER2001-41, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.