CBR1 Rabbit Polyclonal Antibody
cat.: ER2001-45
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 30 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human CBR1 aa 220-277. |
| Positive control: | SK-BR-3 (Human breast cancer cell) cell lysate, A549 (Human lung adenocarcinoma cell) cell lysate, MCF7 (Human breast cancer cell) cell lysate, rat testis tissue, human liver carcinoma tissue, human skin tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:5,000 1:200-1:1,000 |
| Uniprot #: | SwissProt: P16152 Human | P47727 Rat |
| Alternative names: | 15 hydroxyprostaglandin dehydrogenase [NADP+] 15-hydroxyprostaglandin dehydrogenase [NADP+] Carbonyl reductase [NADPH] 1 Carbonyl Reductase 1 CBR 1 CBR1 CBR1_HUMAN CRN NADPH dependent carbonyl reductase 1 NADPH-dependent carbonyl reductase 1 Prostaglandin 9 ketoreductase Prostaglandin 9-ketoreductase Prostaglandin E(2) 9 reductase Prostaglandin-E(2) 9-reductase SDR21C1 |
Images
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Fig1:
Western blot analysis of CBR1 on different lysates with Rabbit anti-CBR1 antibody (ER2001-45) at 1/5,000 dilution. Lane 1: SK-BR-3 (Human breast cancer cell) cell lysate Lane 2: A549 (Human lung adenocarcinoma cell) cell lysate Lane 3: MCF7 (Human breast cancer cell) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER2001-45, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 30 kDa Observed band size: 30 kDa |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-CBR1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-45, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-CBR1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-45, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-CBR1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-45, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.