CBR1 Rabbit Polyclonal Antibody
cat.: ER2001-45
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Rat
Applications: WB, IHC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/ml.
Purification: Protein A affinity purified.
Molecular weight: 30 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human CBR1 aa 220-277.
Positive control: SKBR-3 cell lysate, A549 cell lysate, Siha cell lysate, rat testis tissue, human liver carcinoma tissue, human skin tissue.
Subcellular location: Cytoplasm.
Recommended Dilutions:
  WB
  IHC

1:500-1:1,000
1:200-1:1,000
Uniprot #: SwissProt: P16152 Human | P47727 Rat
Alternative names: 15 hydroxyprostaglandin dehydrogenase [NADP+] 15-hydroxyprostaglandin dehydrogenase [NADP+] Carbonyl reductase [NADPH] 1 Carbonyl Reductase 1 CBR 1 CBR1 CBR1_HUMAN CRN NADPH dependent carbonyl reductase 1 NADPH-dependent carbonyl reductase 1 Prostaglandin 9 ketoreductase Prostaglandin 9-ketoreductase Prostaglandin E(2) 9 reductase Prostaglandin-E(2) 9-reductase SDR21C1
Images
ER2001-45_1.jpg Fig1: Western blot analysis of CBR1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-45, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: SKBR-3 cell lysate
Lane 2: A549 cell lysate
Lane 3: Siha cell lysate
ER2001-45_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-CBR1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-45, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER2001-45_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-CBR1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-45, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER2001-45_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-CBR1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-45, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.