ER2001-50

SSB Rabbit Polyclonal Antibody

cat.: ER2001-50

Product Type:	Rabbit polyclonal IgG, primary antibodies
Species reactivity:	Human
Applications:	WB, IF-Cell, IHC-P, FC
Clonality:	Polyclonal

Form:	Liquid
Storage condition:	Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer:	1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Immunogen affinity purified.
Molecular weight:	Predicted band size: 47 kDa
Isotype:	IgG
Immunogen:	Recombinant protein within human SSB aa 150-350.
Positive control:	Daudi (Human Burkitt's lymphoma cell) cell lysate, Ramos (Human Burkitt's lymphoma cell) cell lysate, Raji (Human Burkitt's lymphoma cell) cell lysate, K-562 (Human chronic myelogenous leukemia cell) cell lysate, JAR, human pancreas tissue, human stomach cancer tissue, human kidney tissue, human thyroid tissue, HCT116.
Subcellular location:	Nucleus.
Recommended Dilutions: WB IF-Cell IHC-P FC	1:5,000 1:50-1:200 1:100-1:500 1:50-1:100
Uniprot #:	SwissProt: P05455 Human
Alternative names:	Autoantigen La La La autoantigen La autoantigen homolog La protein La ribonucleoprotein La ribonucleoprotein domain family member 3 LA_HUMAN LARP3 Lupus La antigen Lupus La protein Lupus La protein homolog MGC118101 MGC93380 mRNA for autoantigen OTTMUSP00000014043 RP23 273G23.1 Sjoegren syndrome type B antigen Sjogren syndrome antigen B (autoantigen La) Sjogren syndrome antigen B SS B SS-B SS-B/La protein SSB

Images

	Fig1: Western blot analysis of SSB on different lysates with Rabbit anti-SSB antibody (ER2001-50) at 1/5,000 dilution. Lane 1: Daudi (Human Burkitt's lymphoma cell) cell lysate Lane 2: Ramos (Human Burkitt's lymphoma cell) cell lysate Lane 3: Raji (Human Burkitt's lymphoma cell) cell lysate Lane 4: K-562 (Human chronic myelogenous leukemia cell) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 4 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER2001-50, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 47 kDa Observed band size: 50 kDa
	Fig2: ICC staining of SSB in JAR cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER2001-50, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
	Fig3: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-SSB antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ER2001-50, 1/500) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig4: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-SSB antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ER2001-50, 1/500) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-SSB antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ER2001-50, 1/500) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig6: Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-SSB antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ER2001-50, 1/500) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig7: Flow cytometric analysis of SSB was done on HCT116 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER2001-50, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.