Anti-p38 (phospho T180) antibody
cat.: ER2001-51
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Peptide affinity purified.
Molecular weight: 41 kDa
Isotype: IgG
Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Thr180 of human P38.
Positive control: 293 cell lysate untreated with UV, 293 cell lysate treated with UV for 60 minutes, A431 cell lysate untreated with UV, A431 cell lysate treated with UV for 40 minutes, Hela cell lysate, Hela cell lysate treated with anisomycin at 250 ng/ml for 30 minutes, Hela cell lysate treated with UV for 40 minutes.
Subcellular location: Cytoplasm, Nucleus.
Recommended Dilutions:
  WB

1:500-1:1,000
Uniprot #: SwissProt: Q16539 Human
Alternative names: CSAID Binding Protein 1 CSAID binding protein CSAID-binding protein Csaids binding protein CSBP 1 CSBP 2 CSBP CSBP1 CSBP2 CSPB1 Cytokine suppressive anti-inflammatory drug-binding protein EXIP MAP kinase 14 MAP kinase MXI2 MAP kinase p38 alpha MAPK 14 MAPK14 MAX interacting protein 2 MAX-interacting protein 2 Mitogen Activated Protein Kinase 14 Mitogen activated protein kinase p38 alpha Mitogen-activated protein kinase 14 Mitogen-activated protein kinase p38 alpha MK14_HUMAN Mxi 2 MXI2 p38 ALPHA p38 p38 MAP kinase p38 MAPK p38 mitogen activated protein kinase p38ALPHA p38alpha Exip PRKM14 PRKM15 RK SAPK2A Stress-activated protein kinase 2a
Images
ER2001-51_1.jpg Fig1: Western blot analysis of p38 (phospho T180) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-51, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: 293 cell lysate, untreated with UV
Lane 2: 293 cell lysate, treated with UV for 60 minutes
ER2001-51_2.jpg Fig2: Western blot analysis of p38 (phospho T180) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-51, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: A431 cell lysate, untreated with UV
Lane 2: A431 cell lysate, treated with UV for 40 minutes
ER2001-51_3.jpg Fig3: Western blot analysis of p38 (phospho T180) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-51, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Hela cell lysate, untreated
Lane 2: Hela cell lysate, treated with anisomycin at 250 ng/ml for 30 minutes
Lane 3: Hela cell lysate, treated with UV for 40 minutes
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.