Phospho-P38 (Thr180 + Tyr182) Rabbit Polyclonal Antibody
cat.: ER2001-52
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1 mg/mL. |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 41 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic phospho-peptide corresponding to residues surrounding Thr180 and Y182 of human P38. |
| Positive control: | 293 cell lysate untreated with UV, 293 cell lysate treated with UV for 60 minutes, A431 cell lysate untreated with UV, A431 cell lysate treated with UV for 40 minutes, Hela cell lysate, Hela cell lysate treated with UV for 40 minutes, Hela cell lysate treated with anisomycin at 250 ng/ml for 30 minutes. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB |
1:500-1:2,000 |
| Uniprot #: | SwissProt: Q16539 Human |
| Alternative names: | CSAID Binding Protein 1 CSAID binding protein CSAID-binding protein Csaids binding protein CSBP 1 CSBP 2 CSBP CSBP1 CSBP2 CSPB1 Cytokine suppressive anti-inflammatory drug-binding protein EXIP MAP kinase 14 MAP kinase MXI2 MAP kinase p38 alpha MAPK 14 MAPK14 MAX interacting protein 2 MAX-interacting protein 2 Mitogen Activated Protein Kinase 14 Mitogen activated protein kinase p38 alpha Mitogen-activated protein kinase 14 Mitogen-activated protein kinase p38 alpha MK14_HUMAN Mxi 2 MXI2 p38 ALPHA p38 p38 MAP kinase p38 MAPK p38 mitogen activated protein kinase p38ALPHA p38alpha Exip PRKM14 PRKM15 RK SAPK2A Stress-activated protein kinase 2a |
Images
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Fig1:
Western blot analysis of Phospho-P38 (Thr180 + Tyr182) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-52, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: 293 cell lysate, untreated with UV Lane 2: 293 cell lysate, treated with UV for 60 minutes |
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Fig2:
Western blot analysis of Phospho-P38 (Thr180 + Tyr182) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-52, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A431 cell lysate, untreated with UV Lane 2: A431 cell lysate, treated with UV for 40 minutes |
|
Fig3:
Western blot analysis of Phospho-P38 (Thr180 + Tyr182) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-52, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate, untreated Lane 2: Hela cell lysate, treated with UV for 40 minutes Lane 3: Hela cell lysate, treated with anisomycin at 250 ng/ml for 30 minutes |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.