Anti-Transferrin antibody
cat.: ER2001-53
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IHC, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A purified.
Molecular weight: 77 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human Transferrin aa 500-550.
Positive control: Human liver tissue, HepG2 cell lysates, HepG2.
Subcellular location: Secreted.
Recommended Dilutions:
  WB
  IHC
  FC

1:500-1:5,000
1:100-1:500
1:50-1:100
Uniprot #: SwissProt: P02787 Human
Alternative names: Apotransferrin antibody Beta 1 metal binding globulin antibody Beta-1 metal-binding globulin antibody DKFZp781D0156 antibody PRO1400 antibody PRO1557 antibody PRO2086 antibody Serotransferrin antibody Serotransferrin precursor antibody Siderophilin antibody TF antibody TFQTL1 antibody Transferin antibody Transferrin antibody TRFE_HUMAN antibody
Images
ER2001-53_1.jpg Fig1: Western blot analysis of Transferrin on HepG2 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-53, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ER2001-53_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Transferrin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-53, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER2001-53_3.jpg Fig3: Flow cytometric analysis of Transferrin was done on HepG2 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER2001-53, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.