eIF-6 Rabbit Polyclonal Antibody
cat.: ER2001-54
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 26 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human eIF-6 aa 1-200. |
| Positive control: | SHSY5Y cell lysate, A549 cell lysate, HepG2 cell lysate, human colon tissue, mouse colon tissue, SW620. |
| Subcellular location: | Centromere, Chromosome, Nucleus. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:1,000 1:500-1:2,000 1:50-1:100 |
| Uniprot #: | SwissProt: P56537 Human | O55135 Mouse |
| Alternative names: | b(2)gcn B(2)GCN homolog B4 integrin interactor Binding protein of beta-4 integrin CAB eIF-6 EIF3A EIF6 Eukaryotic translation initiation factor 3A Eukaryotic translation initiation factor 6 IF6_HUMAN Integrin beta 4 binding protein ITGB4BP OK/SW-cl.27 p27 beta 4 integrin binding protein p27(BBP) p27BBP RP4-614O4.1 |
Images
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Fig1: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-eIF-6 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-54, 1/2,000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig2: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-eIF-6 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-54, 1/2,000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Flow cytometric analysis of eIF-6 was done on SW620 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER2001-54, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig4:
Western blot analysis of eIF-6 on different lysates with Rabbit anti-eIF-6 antibody (ER2001-54) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: K-562 cell lysate Lane 4: C2C12 cell lysate Lane 5: Mouse liver tissue lysate Lysates/proteins at 20 µg/Lane. Exposure time: 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER2001-54, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 27 kDa Observed band size: 27 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.