Clusterin Rabbit Polyclonal Antibody
cat.: ER2001-58
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 52 kDa. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Clusterin aa350-449. |
| Positive control: | Hela cell lysate, SKBR-3 cell lysate, human tonsil tissue, human pancreas tissue, A549. |
| Subcellular location: | Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Membrane, Microsome, Mitochondrion, Nucleus, Secreted. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:1,000 1:100-1:500 1:50-1:100 |
| Uniprot #: | SwissProt: P10909 Human |
| Alternative names: | 40 AAG 4 AAG4 Aging associated protein 4 Aging-associated gene 4 protein AI893575 APO J Apo-J APOJ ApoJalpha ApoJbeta Apolipoprotein J ApolipoproteinJ CLI CLU CLU1 CLU2 CLUS_HUMAN Clusterin alpha chain Clusterin Clusterin beta chain Complement associated protein SP 40 40 Complement associated protein SP 40 Complement associated protein SP40 Complement cytolysis inhibitor a chain Complement cytolysis inhibitor Complement cytolysis inhibitor b chain Complement lysis inhibitor Complement-associated protein SP-40 D14Ucla3 Dimeric acid glycoprotein Glycoprotein 80 Glycoprotein III GP80 Ku70-binding protein 1 KUB 1 KUB1 MGC24903 NA1/NA2 RATTRPM2B SGP 2 SGP2 SP 40 SP40 Sugp-2 Sulfated glycoprotein 2 Testosterone repressed prostate message 2 Testosterone-repressed prostate message 2 TRPM 2 TRPM-2 TRPM2 TRPM2B Trpmb |
Images
|
Fig1:
Western blot analysis of Clusterin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-58, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: SKBR-3 cell lysate |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Clusterin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-58, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Clusterin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-58, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4: Flow cytometric analysis of Clusterin was done on A549 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER2001-58, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.