TREM2 Rabbit Polyclonal Antibody
cat.: ER2001-59
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 25 kDa. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Trem2 aa 180-230. |
| Positive control: | THP-1 cell lysate, rat bone marrow tissue lysate. |
| Subcellular location: | Cell membrane, Membrane, Secreted. |
| Recommended Dilutions:
WB FC |
1:500 1:50-1:100 |
| Uniprot #: | SwissProt: Q9NZC2 Human |
| Alternative names: | TREM 2 TREM-2 TREM2 TREM2_HUMAN TREM2a TREM2b TREM2c Trggering receptor expressed on myeloid cells 2 Trggering receptor expressed on myeloid cells 2a Triggering receptor expressed on monocytes 2 Triggering receptor expressed on myeloid cells 2 |
Images
|
Fig1:
Western blot analysis of TREM2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-59, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: THP-1 cell lysate Lane 2: Rat bone marrow tissue lysate |
|
Fig2: Flow cytometric analysis of TREM2 was done on THP-1 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER2001-59, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.