Dux Rabbit Polyclonal Antibody
cat.: ER2001-60
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | WB, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 74 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within mouse Dux aa 520-570. |
| Positive control: | Non-transfected 293T cells lysate, His-tagged Dux transfected 293T cells lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IF-Cell |
1:500 1:50-1:200 |
| Uniprot #: | SwissProt: A1JVI8 Mouse |
| Alternative names: | Double homeobox 4 Double homeobox protein 10 Double homeobox protein 4 Double homeobox protein 4/10 DUX10 DUX4L DUX4L1 |
Images
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Fig1:
Western blot analysis of Dux on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-60, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Non-transfected 293T cells lysate Lane 2: His-tagged Dux transfected 293T cells lysate |
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Fig2: ICC staining of Dux in Non-transfected 293T and His-tagged Dux transfected 293T cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER2001-60, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.