CBL Rabbit Polyclonal Antibody
cat.: ER2001-61
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 100 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human C-CBL aa 580-630. |
| Positive control: | 293T cell lysates. |
| Subcellular location: | Cell membrane, Cell projection, Cytoplasm, Golgi apparatus, Membrane. |
| Recommended Dilutions:
WB |
1:500 |
| Uniprot #: | SwissProt: P22681 Human |
| Alternative names: | 4732447J05Rik C CBL Cas Br M (murine) ecotropic retroviral transforming sequence Casitas B lineage lymphoma proto oncogene Casitas B-lineage lymphoma proto-oncogene CBL 2 cbl CBL_HUMAN CBL2 E3 ubiquitin protein ligase CBL E3 ubiquitin-protein ligase CBL Oncogene CBL2 Proto oncogene c CBL Proto-oncogene c-CBL RGD1561386 RING finger protein 55 RNF55 Signal transduction protein CBL |
Images
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Fig1:
Western blot analysis of CBL on 293T cell lysates with Rabbit anti-CBL antibody (ER2001-61) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 100 kDa Observed band size: 110/70 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER2001-61) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.