CBL Rabbit Polyclonal Antibody
cat.: ER2001-61
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 100 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human C-CBL aa 580-630. |
| Positive control: | Raji cell lysate, 293T cell lysate, THP-1 cell lysate, Jurkat cell lysate. |
| Subcellular location: | Cytoplasm, Cell membrane, Cell projection, cilium, Golgi apparatus. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P22681 Human |
| Alternative names: | 4732447J05Rik C CBL Cas Br M (murine) ecotropic retroviral transforming sequence Casitas B lineage lymphoma proto oncogene Casitas B-lineage lymphoma proto-oncogene CBL 2 cbl CBL_HUMAN CBL2 E3 ubiquitin protein ligase CBL E3 ubiquitin-protein ligase CBL Oncogene CBL2 Proto oncogene c CBL Proto-oncogene c-CBL RGD1561386 RING finger protein 55 RNF55 Signal transduction protein CBL |
Images
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Fig1:
Western blot analysis of CBL on different lysates with Rabbit anti-CBL antibody (ER2001-61) at 1/1,000 dilution. Lane 1: Raji cell lysate Lane 2: 293T cell lysate Lane 3: THP-1 cell lysate Lane 5: Jurkat cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 100 kDa Observed band size: 100 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER2001-61) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.