MTHFD2 Rabbit Polyclonal Antibody
cat.: ER2001-62
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Peptide affinity purified.
Molecular weight: 38 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human MTHFD2 aa 300-350.
Positive control: 293 cell lysates, Daudi.
Subcellular location: Mitochondrion.
Recommended Dilutions:
  WB
  FC

1:500
1:50-1:100
Uniprot #: SwissProt: P13995 Human
Alternative names: Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial Descriptions Methenyltetrahydrofolate cyclohydrolase methylene tetrahydrofolate dehydrogenase (NAD+ dependent) methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2 methylenetetrahydrofolate dehydrogenase (NADP+ dependent) methylenetetrahydrofolate dehydrogenase 2 MGC82516 MTDC_HUMAN MTHFD2 mthfd2 methylene tetrahydrofolate dehydrogenase (NAD+ dependent) NAD-dependent methylene tetrahydrofolate dehydrogenase NAD-dependent methylenetetrahydrofolate dehydrogenase NMDMC
Images
ER2001-62_1.jpg Fig1: Western blot analysis of MTHFD2 on 293 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-62, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ER2001-62_2.jpg Fig2: Flow cytometric analysis of MTHFD2 was done on Daudi cells. The cells were fixed, permeabilized and stained with the primary antibody (ER2001-62, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.