|Product Type:||Rabbit polyclonal IgG, primary antibodies|
|Species reactivity:||Human, Mouse, Rat|
Western blot analysis of RanBP9 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-63, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Lane 1: SKBR-3 cell lysate
Lane 2: 293 cell lysate
Lane 3: Mouse lung tissue lysate
Lane 4: Rat brain tissue lysate
|Fig2: Flow cytometric analysis of RanBP9 was done on F9 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER2001-63, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).|