Anti-RanBP9 antibody
cat.: ER2001-63
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein affinity purified.
Molecular weight: 116 kDa
Isotype: IgG
Immunogen: Recombinant protein within human RanBP9 aa 800-1041.
Positive control: SKBR-3 cell lysate, 293 cell lysate, mouse lung tissue lysate, rat brain tissue lysate, F9.
Subcellular location: Cytoplasm, Nucleus.
Recommended Dilutions:
  WB
  FC

1:500-1:2,000
1:50-1:100
Uniprot #: SwissProt: Q96P70 Human | Q91YE6 Mouse
Alternative names: Imp9 Imp9a Imp9b Importin-9 Importin-9a Ipo9 IPO9_HUMAN Ran-binding protein 9 RanBP9
Images
ER2001-63_1.jpg Fig1: Western blot analysis of RanBP9 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-63, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: SKBR-3 cell lysate
Lane 2: 293 cell lysate
Lane 3: Mouse lung tissue lysate
Lane 4: Rat brain tissue lysate
ER2001-63_2.jpg Fig2: Flow cytometric analysis of RanBP9 was done on F9 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER2001-63, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.