MVK Rabbit Polyclonal Antibody
cat.: ER2001-64
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 42 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human MVK aa 200-396. |
| Positive control: | HeLa (Human cervical adenocarcinoma cell) cell lysate, Hep G2 (Human hepatocellular carcinoma cell) cell lysate, A431 (Human epidermoid carcinoma skin squamous cell) cell lysate, HepG2. |
| Subcellular location: | Cytoplasm, Peroxisome. |
| Recommended Dilutions:
WB FC |
1:2,000 1:50-1:100 |
| Uniprot #: | SwissProt: Q03426 Human |
| Alternative names: | FLJ96772 KIME_HUMAN LH receptor mRNA binding protein LRBP Mevalonate kinase 1 Mevalonate kinase Mevalonic aciduria MK mvk MVLK POROK3 |
Images
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Fig1:
Western blot analysis of MVK on different lysates with Rabbit anti-MVK antibody (ER2001-64) at 1/2,000 dilution. Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 2: Hep G2 (Human hepatocellular carcinoma cell) cell lysate Lane 3: A431 (Human epidermoid carcinoma skin squamous cell) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER2001-64, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 42 kDa Observed band size: 45 kDa |
|
Fig2: Flow cytometric analysis of MVK was done on HepG2 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER2001-64, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.