Pleckstrin Rabbit Polyclonal Antibody
cat.: ER2001-67
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 40 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Pleckstrin aa 300-350. |
| Positive control: | U937 cell lysate, rat spleen tissue lysate, mouse brain tissue lysate. |
| Subcellular location: | Cytosol, extracellular region, plasma membrane, ruffle membrane, cytoplasm, membrane. |
| Recommended Dilutions:
WB |
1:500-1:1,000 |
| Uniprot #: | SwissProt: P08567 Human | Q9JHK5 Mouse | Q4KM33 Rat |
| Alternative names: | FLJ27168 OTTHUMP00000159962 p47 Platelet 47 kDa protein Platelet and leukocyte C kinase substrate Platelet P47 Platelet p47 protein Pleckstrin PLEK PLEK_HUMAN |
Images
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Fig1:
Western blot analysis of Pleckstrin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-67, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: U937 cell lysate Lane 2: Rat spleen tissue lysate Lane 2: Mouse brain tissue lysate |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.