PSMA5 Rabbit Polyclonal Antibody
cat.: ER2001-68
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 26 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human PSMA5 aa 1-200. |
| Positive control: | Hela cell lysate, 293 cell lysate, mouse cerebellum tissue lysate, mouse liver tissue lysate, rat liver tissue lysate, human liver carcinoma tissue, human colon carcinoma tissue. |
| Subcellular location: | Cytoplasm, Nucleus, Proteasome. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:1,000 1:100-1:500 |
| Uniprot #: | SwissProt: P28066 Human | Q9Z2U1 Mouse | P34064 Rat |
| Alternative names: | Macropain zeta chain Multicatalytic endopeptidase complex zeta chain Proteasome (prosome macropain) subunit alpha type 5 Proteasome alpha5 subunit Proteasome component 5 Proteasome subunit alpha type 5 Proteasome subunit alpha type-5 Proteasome subunit zeta Proteasome zeta chain PSA5_HUMAN PSC5 PSMA5 ZETA |
Images
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Fig1:
Western blot analysis of PSMA5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-68, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: 293 cell lysate Lane 3: Mouse cerebellum tissue lysate Lane 4: Mouse liver tissue lysate Lane 5: Rat liver tissue lysate |
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Fig2: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-PSMA5 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-68, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-PSMA5 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-68, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.